Construction and identification of prokaryotic and eukaryotic expression vectors of ScFv-C_ κ of monoclonal antibody against Hantaanvirus NP antigen 抗汉滩病毒NP抗原mAb的ScFv-Cκ基因真核和原核表达载体的构建及鉴定
Methods Immunohistochemistry combined with computer assisted image analysis system were carried out to examine the expressions of factor ⅷ related antigen, bFGF and PCNA in 18 normal prostates and 62 specimens of BPH. 方法应用免疫组化结合计算机图像分析方法检测第Ⅷ因子相关抗原、bFGF和PCNA在18例正常前列腺(NP)和62例BPH中的表达及其相关关系。
Aim To make ELISA typing of sera from HFRS patients from partial districts of Hebei and Shaanxi Provinces using hantavirus nucleocapsid protein ( NP) as antigen. 目的以汉坦病毒重组核衣壳蛋白(NP)为抗原,对陕西、河北两省部分地区HFRS患者的血清进行ELISA分型。
With the rNP as antigen, two hybridomas secreting anti NP monoclonal antibodies persistently and stably were obtained. 以此重组NP作为抗原制备抗NP单克隆抗体,获得两株持续稳定分泌单克隆抗体的杂交瘤细胞株。
The preparation and preliminary application of the McAb anti-NP will contribute to the farther improvement of the dectection of avian influenza's antigen and to provide a useful tool and assay for diagnosis of AI, and even of human-avian influenza. 通过制备和初步应用禽流感病毒NP蛋白特异性单克隆抗体,为进一步临床应用打下基础,为禽流感乃至人禽流感的诊断提供一种有用试剂和快速、准确、实用的诊断方法。
Therefore NP could be regarded as an ideal antigen for diagnosis. 因此,核蛋白可以用作理想的诊断抗原。